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DNA Mutagenesis Random Mutagenesis Kits (隨機突變分析套組)
Within
three billion years of evolution, nature has produced a plethora of proteins
simply by repeated cycles of random mutagenesis followed by in vivo selection
for superior function of the encoded proteins. This example of natural evolution
has guided researchers within the last two decades to develop strategies for in
vitro permutation of proteins.Among the variety of strategies applied, three
major powerful techniques have emerged. dNTP-Mutagenesis、Error-Prone、DNA-Shuffling Cat. No. PP-101 JBS dNTP-Mutagenesis Kit 15 reactions Store
at -20°C, avoid frequent thawing and freezing JBS dNTP-Mutagenesis Kit is based on the incorporation of the mutagenic dNTP analogs 8-oxo-dGTP and dPTP into an amplified DNA fragment by PCR. The mutagenic dNTPs are eliminated by a second PCR step in the presence of the four natural dNTPs only, resulting in a rate of mutagenesis of up to 20%. Cat.No. PP-102 JBS Error-Prone Kit 15 reactions Store
at -20°C, avoid frequent thawing and freezing JBS Error-Prone Kit introduces mutations in the gene of interest using modified PCR reaction conditions. An increased MgCl2 concentration, the addition of MnCl2 and the unbalanced ratio of dNTPs induce an increased error-rate of the DNA-polymerase. The rate of mutagenesis achieved by error-prone PCR is in the range of 0.6-2.0%. Cat.No. PP-103 JBS DNA-Shuffling Kit 15 reactions Store
at -20°C, avoid frequent thawing and freezing JBS
DNA-Shuffling Kit is designed to
generate gene libraries by random fragmentation of one gene or a pool of related
genes. A controlled fragmentation of the DNA is followed by a random reassembly
in a self-priming PCR reaction. This method allows the recombination of
sequences from different, related genes. The overall rate of mutagenesis is
approx. 0.7%. 訂購專線：07-5542589 ; 0800 231 233 For
inquiries or further information, please contact us at service@fomobio.com
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