台灣轉錄科技有限公司     分生器材網

UniPOL - Long Range PCR

high yield : 5-20 kb  

產地：丹麥

Ampliqon UniPOL Enzyme mix, combines Ampliqon’s high quality Taq DNA Polymerase with a small amount of AccuPOL, which exhibit 3’ to 5’ proofreading exonuclease activity. The UniPOL Enzyme mix is optimised for amplifications of 5-20 kb, with high yield.

Key Features

• The choice for amplification of long targets
• High performance thermostable DNA polymerases
• Ideal for rich amplifications
• Ideal for amplifications of targets which require increased sensitivity

Amplification using Taq DNA Polymerase is generally limited to amplification of up to 4-6 kb, depending on template. This is partly due to the lack of 3’ to 5’ proofreading exonuclease activity of the Taq DNA Polymerase.  Mis-incorporation of nucleotides, often lead to processive mistake or a terminal event will occur and the elongation will arrest.  A blend of the processive Taq DNA Polymerase and the AccuPOL proofreading enzyme, that corrects mis-incoporated nucleotides, increase the length of amplification product.
Due to differences in complexity of template, Ampliqon has optimized two different buffers- UniPOL Buffer A and UniPOL Buffer B. Both buffer systems is included in the kit.