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PCR Cloning Kit
Cat.No. RC001
Cloning Vector Kit
20 reactions/kit
Cloning Vector (40 µl, 25ng/ µl)
Control insert DNA(5ng/µl) (10 µl)
T4 DNA ligase(3U/µl) (20 µl)
T4 DNA Ligase B uffer A (100 µl)
T4 DNA Ligase Buffer B (100 µl)
Forward primer(M13-F) (10 µM)(50 µl)
Reverse primer(M13-R) (10µM)(50 µl)
Cat.No. RC013
Cloning Vector only
Cloning Vector (25ng/µl;40µl)
The Vector System is ideal
for cloning PCR products which is add
a single terminal 3'-dA nucleotide overhang (Taq) for rapid cloning and the
ligases may be used
directly to transform competent cells or purified to achieve
a higher efficiency of transformation and get lower background cloning.
Applications
-Terminal 3'-dA nucleotide overhang PCR products
-Transform ligation product into competent cells
-LacZ Complementation for Blue/White screening
-Ampicillin marker for antibiotic selection
-Universal primer for transformation and screening
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