TaqDNA Polymerase 

TaqDNA Polymerase 

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Taq DNA Polymerase is a standard thermostable recombinant DNA polymerase, which exhibits very high performance in primer extension and other molecular biology applications.  Ampliqon Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity.
The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). The choice for high performance.

Key Features

  • The choice when high-fidelity is not required

  • High performance thermostable DNA polymerase

  • Ideal for rich amplifications

  • Optimal for TA-cloning

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dryTAQTM DNA Polymerase

Ampliqon dryTAQTM DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications.

Ampliqon dryTAQTM DNA Polymerase contains a stabilizer which makes the enzyme completely stable at room temperature for at least six month. Using a special evaporation process the enzyme has been crystallized in the reaction tubes. 

Key Features

  • The choice when high-fidelity is not required

  • High performance thermostable DNA polymerase

  • Ideal for rich amplifications

  • Optimal for TA-cloning

  • Can be shipped at room-temperature with-out loosing performance

Ampliqon dryTAQTM DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications.

Ampliqon dryTAQTM DNA Polymerase contains a stabilizer which makes the enzyme completely stable at room temperature for at least six month. Using a special evaporation process the enzyme has been crystallized in the reaction tubes.

Ampliqon dryTAQTM DNA Polymerase has both a 5'→3' DNA polymerase and a 5'→3' exonuclease activity. The enzyme lacks a 3'→5' exonuclease activity and has no proofreading ability. dryTAQTM DNA Polymerase leaves an A′ overhang, which makes the enzyme ideal for TA cloning.

Component

Vol./reaction

Final Conc.

10X Buffer

5 μL

1X

dNTP mix(12.5 mM of each)

0.8 μL

0.2 mM of each dNTP

Primer A

Variable

0.1?.0 μM

Primer B

Variable

0.1?.0 μM

Taq DNA Polymerase

Variable

1-5 units

Distilled Water

Variable

- - - -

Template DNA

Variable

Variable

TOTAL volume

50 μL

- - - -

 

900003    500 units