定量PCR,Real Time PCR, RealTime PCR, qPCR mastermix, RealQ Master mix

aRealQ– Real Time PCR Master Mix Kit

1.25ml x 2(SYBR Green dye)=2.5ml (4500)

1.25ml x 4(SYBR Green dye)=5ml (8000)

1.25ml x 2(Probe專用不含 dye)=2.5ml (4500)

1.25ml x 4(Probe專用不含 dye)=5ml (8000)

適合ABI PRISM, LightCycler, i-Cycler, Smart-Cycler, Rotor-Gene

(丹麥Ampliqon)

Ampliqon has developed the RealQ PCR Master Mix, a single-tube 2X reagent ideal for most quantitative real time PCR applications.  The RealQ PCR kit includes the components necessary for performing PCR amplification, and have been successfully used for amplification and detection a variety of DNA target such as genomic DNA, cDNA and plasmid DNA. The RealQ PCR Master Mix includes the TEMPase Hot Start DNA polymerase, a modified Taq DNA polymerase with hot start capabilities. The TEMPase Hot Start enzyme improves the PCR amplification reaction by decreasing background from non-specific amplification and increases amplification of desired products.

Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of SNP or quantitative gene transcripts. The use of fluorescent probe technologies reduces the risk of sample contamination while maintaining convenience, speed and high throughput screening capabilities. Ampliqon has developed the RealQ-PCR Master Mix, a single-tube 2X reagent ideal for most Quantitative PCR applications. The RealQ-PCR kit support quantitative amplification and detection with multiplex capability. The RealQ-PCR kit has been designed for optimal performance on ABI PRISMTM Instruments, the LightCyclerTM Instrument, the Mx4000TM Instrument and the DNA Engine OpticonTM System. The RealQ-PCR kits includes the components necessary for performing PCR amplification, and have been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA and plasmid DNA.

The RealQ-PCR master mix includes the TEMPase Hot Start DNA polymerase, a modified Taq DNA polymerase with hot start capabilities. The TEMPase Hot Start enzyme improves the PCR amplification reaction by decreasing background from non-specific amplification and increases amplification of desired products.